rabbit anti caii antibody  (Santa Cruz Biotechnology)

Journal: Scientific Reports

Article Title: Development of type I/II oligodendrocytes regulated by teneurin-4 in the murine spinal cord

doi: 10.1038/s41598-020-65485-0

Figure Lengend Snippet: Reduced CAII/CC1-double positive oligodendrocytes in the Ten-4 −/− spinal cord at 7 weeks. ( a ) Immunohistochemical images of CAII (magenta) and CC1 (green) in Ten-4 −/− and WT spinal cords at 7 weeks. CAII/CC1-double positive cells were substantially decreased in the WM in Ten-4 −/− mice. All CAII-positive cells were positive for CC1 and there were 45% of CC1-single positive cells. Scale bar: 200 μm. ( b ) Higher magnification of CAII/CC1-double positive cells in the VFo, VFi, CST, FC, and FG. Double positive cells (white) were reduced in Ten-4 −/− mice. CAII-single positive cells were not observed. Arrows: CAII/CC1-double positive cells. Arrowheads: CC1-single positive cells. Scale bar: 20 μm. ( c ) Quantitative analysis of the number of CC1-single positive and CAII/CC1-double positive cells in the entire WM areas of the spinal cord in WT and Ten-4 −/− mice at 7 weeks. The reduction of CAII/CC1-double positive cells in Ten-4 −/− mice was more prominent than that of CC1-single positive cells. Triplicate experiments were independently performed (n = 3). Error bars represent mean ± s.e.m. The two-tailed Student’s t -test was used for the statistical analysis in the experiments with two groups, WT and Ten-4 −/−. * p < 0.05.

Article Snippet: After blocking with Power Block Universal Blocking Reagent (BioGenex Laboratories) for 1 h at room temperature, primary antibodies, rabbit anti-CAII (1:250; Sigma-Aldrich), mouse anti-APC (clone CC1) (1:500; Millipore), rabbit anti-MBP (1:100; Millipore), and rabbit anti-neurofilament (1:500; Sigma-Aldrich), in 1% bovine serum albumin (BSA) in PBS, were incubated overnight at 4 °C.

Techniques: Immunohistochemical staining, Two Tailed Test

Journal: Scientific Reports

Article Title: Development of type I/II oligodendrocytes regulated by teneurin-4 in the murine spinal cord

doi: 10.1038/s41598-020-65485-0

Figure Lengend Snippet: Reduction of CAII/CC1-double positive type I/II oligodendrocytes in Ten-4 −/− mice at the postnatal stage. ( a ) Immunohistochemical images of CAII (magenta) and CC1 (green) at P11 of Ten-4 −/− and WT mice are displayed. CAII/CC1-double positive cells (white) were decreased in the VFi, CST, and FG in Ten-4 −/− mice. CAII-single positive cells were rarely observed both in Ten-4 −/− and WT mice. Arrows: CAII/CC1-double positive cells. Arrowheads: CC1-single positive cells. Scale bar: 20 μm. ( b ) Quantitative analysis of the number of the CC1-single positive and CAII/CC1-double positive cells in the entire WM areas of the spinal cord in WT and Ten-4 −/− mice at P11. The reduction of CAII/CC1-double positive type I/II oligodendrocytes in Ten-4 −/− mice was already observed at P11. Triplicate experiments were independently performed (n = 3). Error bars represent mean ± s.e.m. The two-tailed Student’s t -test was used for the statistical analysis in the experiments with two groups, WT and Ten-4 −/−. * p < 0.05.

Article Snippet: After blocking with Power Block Universal Blocking Reagent (BioGenex Laboratories) for 1 h at room temperature, primary antibodies, rabbit anti-CAII (1:250; Sigma-Aldrich), mouse anti-APC (clone CC1) (1:500; Millipore), rabbit anti-MBP (1:100; Millipore), and rabbit anti-neurofilament (1:500; Sigma-Aldrich), in 1% bovine serum albumin (BSA) in PBS, were incubated overnight at 4 °C.

Techniques: Immunohistochemical staining, Two Tailed Test

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men.

doi: 10.1152/japplphysiol.00630.2016

Figure Lengend Snippet: Fig. 3. Basigin antibody validation. Using Western blotting, two duplicate gels (1 and 2) were loaded with four lanes of a single sample of skeletal muscle homogenate (M), six differ- ent concentrations of an internal standard (IS1– IS6: 8–38 g protein), and one lane of a Hep G2 cell lysate positive control (ve). Follow- ing transfer, membrane 1A was immunoblotted with a mouse monoclonal anti-basigin antibody (sc-21746), and membrane 2A was immuno- blotted with a different mouse monoclonal anti- basigin antibody (sc-46700). After imaging, the peroxidase of the horseradish peroxidase- conjugated secondary antibodies was inacti- vated by 15 min of H2O2 incubation. Both membranes were reprobed (1B and 2B) with a goat polyclonal anti-basigin antibody (sc- 9757). See text for additional details on immu- noblotting.

Article Snippet: Primary Rabbit polyclonal anti-MCT1 Merck Millipore AB3540P/2136555 1:1,000 1:15,000 Rabbit polyclonal anti-MCT4 Merck Millipore AB3316P/2397059 1:1,000 1:20,000 Mouse monoclonal anti-basigin Santa Cruz sc-21746/K1913 1:200 1:7,500 Mouse monoclonal anti-NHE1 Merck Millipore MAB3140/2283852 1:500 1:7,500 Rabbit polyclonal anti-NBCe1 Cell Signaling 11867/0001 1:500 1:5,000 Rabbit polyclonal anti-CAII Santa Cruz sc-25596/F0611 1:1,250 1:30,000 Mouse monoclonal anti-CAIII Abnova H00000761-M02/12243-S1 1:2,500 1:40,000 Mouse polyclonal anti-CAIV Abnova H00000762-B02P/08325 WULz 1:500 1:10,000 Mouse polyclonal anti-CAXIV Abnova H00023632-B01P/08358 WULz 1:750 1:10,000 Secondary Goat anti-mouse IgG Perkin Elmer NEF822001EA Goat anti-rabbit IgG Perkin Elmer NEF812001EA J Appl Physiol • doi:10.1152/japplphysiol.00630.2016 • www.jappl.org Overall basigin abundance changed in response to training (time main effect: F3,37.2 4.47, P 0.009) (Fig. 4C).

Techniques: Biomarker Discovery, Western Blot, Positive Control, Membrane, Imaging, Incubation